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Effects of Static Cold Storage and Hypothermic Machine Perfusion on Oxidative Stress Factors, Adhesion Molecules, and Zinc Finger Transcription Factor Proteins Before and After Liver Transplantation

De-Fang Zhao, Qin Dong, Tong Zhang

(Department of Hepatobiliary Surgery, The First Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, China (mainland))

Ann Transplant 2017; 22:96-100

DOI: 10.12659/AOT.901897


BACKGROUND: This study aimed to investigate the effects of static cold storage (SCS) and hypothermic machine perfusion (HMP) on the oxidative stress factors (OSF), adhesion molecules (AM), and zinc finger transcription factor (Snail) before and after liver transplantation.
MATERIAL AND METHODS: Experimental dogs were randomly divided into donor (group A), SCS (group B), and HMP (group C) (n=30) groups. Livers retrieved from group A were transplanted into group B after SCS, and the livers sampled from group B were transplanted into group C after HMP. The dogs in group A were euthanized and discarded, and the livers sampled from group C were used for other experiments. Twenty dogs with successful liver transplants were randomly selected from groups B and C for analysis.
RESULTS: During the liver sampling process, the levels of OSF, AM, and Snail between the 2 groups showed no significant differences (P>0.05); before the transplantation, the levels of chemokine CXCL14 and Snail between the 2 groups showed no significant differences (P>0.05), and compared with group B, HIF-1α and P-selectin in group C were lower (P<0.01); 60 min after the transplantation, HIF-1α, chemokine CXCL14, P-selectin, and Snail in group C were lower than that in group B (P<0.01).
CONCLUSIONS: HMP can significantly reduce the levels of OSF and inflammatory factors, which is conducive for liver transplantation.

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