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14 July 2020 : Original article  

Pretreatment with a Phosphodiesterase-3 Inhibitor, Milrinone, Reduces Hepatic Ischemia-Reperfusion Injury, Minimizing Pericentral Zone-Based Liver and Small Intestinal Injury in Rats

Shinichi Nakanuma1ABCDEFG*, Hidehiro Tajima1AC, Hiroyuki Takamura1ACG, Seisho Sakai1A, Ryosuke Gabata1B, Mitsuyoshi Okazaki1B, Hiroyuki Shinbashi1B, Yoshinao Ohbatake1B, Isamu Makino1B, Hironori Hayashi2A, Tomoharu Miyashita1AC, Sachio Fushida1A, Tetsuo Ohta1ACE

DOI: 10.12659/AOT.922306

Ann Transplant 2020; 25:e922306

Figure 1 Experimental protocol: In the ischemia-reperfusion (IR)+milrinone group, milrinone was injected into the spleen before whole hepatic ischemia. In the IR and sham groups, saline was injected into the spleen. After 30 min of whole hepatic ischemia, hepatic reperfusion was initiated. Five hours after the reperfusion or sham operation, the rats were killed to collect blood samples and liver and small intestinal specimens (A). Either milrinone or saline was intraportally administered via intrasplenic injection. The insertion site of the splenic injection was cauterized via electric cautery to stop the bleeding (B).

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Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358