14 July 2020: Original Paper
Pretreatment with a Phosphodiesterase-3 Inhibitor, Milrinone, Reduces Hepatic Ischemia-Reperfusion Injury, Minimizing Pericentral Zone-Based Liver and Small Intestinal Injury in Rats
Shinichi Nakanuma 1ABCDEFG* , Hidehiro Tajima 1AC , Hiroyuki Takamura 1ACG , Seisho Sakai 1A , Ryosuke Gabata 1B , Mitsuyoshi Okazaki 1B , Hiroyuki Shinbashi 1B , Yoshinao Ohbatake 1B , Isamu Makino 1B , Hironori Hayashi 2A , Tomoharu Miyashita 1AC , Sachio Fushida 1A , Tetsuo Ohta 1ACEDOI: 10.12659/AOT.922306
Ann Transplant 2020; 25:e922306
Figure 1 Experimental protocol: In the ischemia-reperfusion (IR)+milrinone group, milrinone was injected into the spleen before whole hepatic ischemia. In the IR and sham groups, saline was injected into the spleen. After 30 min of whole hepatic ischemia, hepatic reperfusion was initiated. Five hours after the reperfusion or sham operation, the rats were killed to collect blood samples and liver and small intestinal specimens (A). Either milrinone or saline was intraportally administered via intrasplenic injection. The insertion site of the splenic injection was cauterized via electric cautery to stop the bleeding (B).