31 October 2023: Original Paper
Three-Dimensional Printing of Polycaprolactone/Nano-Hydroxyapatite Composite Scaffolds with a Pore Size of 300/500 μm is Histocompatible and Promotes Osteogenesis Using Rabbit Cortical Bone Marrow Stem Cells
Yang Yang ABCDEFG , Bing Qiu ABCDEG* , Zhuxing Zhou BCDF , Chaoran Hu BCF , Jia Li BCF , Cheng Zhou BFDOI: 10.12659/AOT.940365
Ann Transplant 2023; 28:e940365
Figure 2 Isolation, culture, and identification of BMSCs-C cells. (A) Morphology of primary bone marrow mesenchymal stem cells (BMSCs) and bone compact mesenchymal stem cells (BMSCs-C) on day 4 (P1) and generation of BMSCs-M and BMSCs-C in P5. (B) Expression of surface biomarkers of BMSCs-C cells (CD29, CD44, CD34, CD45) in P5. (C) Alizarin red staining (Magnification, 400×; red staining) of osteogenic induction, oil red O staining of adipogenic induction (Magnification, 400×; red staining) and Alcian blue staining (Magnification, 400×, blue staining) of chondrogenic induction in BMSCs-C cells. Data analysis was carried out using SPSS Statistics version 22.0 (IBM SPSS, Inc., Chicago, IL, USA).