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24 November 2017 : Original article  

Intrahepatic MicroRNA Profile of Liver Transplant Recipients with Hepatitis C Virus Co-Infected with Human Immunodeficiency Virus

Hisamitsu Miyaaki1ACDEF*, Mitsuhisa Takatsuki2AB, Tatsuki Ichikawa1AD, Masaaki Hidaka2B, Akihiko Soyama2B, Hideki Ohdan3B, Yukihiro Inomata4B, Shinji Uemoto5B, Norihiro Kokudo6B, Kazuhiko Nakao7D, Susumu Eguchi2BG

DOI: 10.12659/AOT.906236

Ann Transplant 2017; 22:701-706

Abstract

BACKGROUND: In patients with human immunodeficiency virus (HIV) and hepatitis C virus (HCV) co-infection, HIV can modulate HCV replication and immune response as well as accelerate liver fibrosis. The role of miRNA in HIV/HCV co-infection is not fully elucidated. The aim of this study was to examine the differential expression of miRNAs in the liver.

MATERIAL AND METHODS: Thirteen patients who had undergone a liver transplant (7 HCV-infected and 6 HIV/HCV-co-infected patients) were examined using a miRNA array containing 1347 human miRNAs. To confirm the microarray results, data for 20 patients (10 HCV-infected and 10 HIV/HCV-co-infected) were validated using real-time polymerase chain reaction probing for miR101b, miR149, and miR200c. This miRNA was selected based on microarray results and its biological significance in liver fibrosis.

RESULTS: Microarray analysis revealed 22 miRNAs that were differentially expressed in the HIV/HCV-co-infected group compared to the HCV-infected group (p<0.05). The expression of miR-101b and miR149 was significantly decreased in the HIV/HCV-co-infected group compared to that in the HCV-infected group (miR101b, 0.103±0.09 vs. 0.0157±0.0093, p=0.007; miR149, 0.152±0.159 vs. 0.0192±0.015, p=0.025).

CONCLUSIONS: HIV/HCV co-infection may promote liver fibrosis by modulating miRNA expression.

Keywords: Hepatitis C Antibodies, Hepatitis, Viral, Human, Liver Cirrhosis

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Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358