Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

24 April 2018 : Original article  

Oxygen Consumption of the Aerobically-Perfused Cardioplegic Donor Heart at Different Temperatures

Guangqi Qin1ABDEF, Yang Su1B, Trygve Sjöberg1ACDEF, Stig Steen1ACDEFG*

DOI: 10.12659/AOT.907753

Ann Transplant 2018; 23:268-273

Abstract

BACKGROUND: The aim of this study was to investigate the oxygen consumption of explanted aerobically-perfused cardioplegic porcine hearts at different temperatures.

MATERIAL AND METHODS: Explanted hearts from 30 pigs weighing 50 kg were randomized into 5 groups. The hearts received continuous antegrade perfusion within a temperature-controlled sealed system. The perfusate consisted of an albumin-containing hyperoncotic cardioplegic nutrition solution with erythrocytes to a hematocrit of 10%. Five temperatures were studied: 37, 30, 22, 15, and 8°C. When the erythrocytes in the perfusate were fully saturated, the oxygenator was excluded from the circuit and blood gases were analyzed periodically until the erythrocytes had desaturated to less than 20%. Between 80% and 60% saturation the desaturation curves were linear in all groups and the oxygen consumption was calculated from this part of the curves.

RESULTS: The weight of the hearts was 208±4 g (mean ±SEM, n=30). The oxygen consumption in mL/min/100 g heart tissue was (mean ±SEM, n=6 in each group) 37°C: 1.10±0.04; 30°C: 0.58±0.02; 22°C: 0.33±0.01; 15°C: 0.21±0.01; and 8°C: 0.16±0.02.

CONCLUSIONS: The oxygen consumption of the cardioplegic perfused pig heart at normothermia was 1.1 mL/min/100 g and was reduced by 85% to 0.16 mL/min/100 g at 8°C.

Keywords: Heart, Metabolism, Organ Preservation, Oxygen Consumption, Perfusion

0 Comments

SARS-CoV-2/COVID-19

29 November 2022 : Editorial  

Editorial: The World Health Organization (WHO) Fungal Priority Pathogens List in Response to Emerging Funga...

Med Sci Monit In Press; DOI: 10.12659/MSM.939088  

15 November 2022 : Original article  

Changes in Physical Activity Due to Fear of COVID-19 and Its Impact on Depression Among Post-Liver Transpla...

Ann Transplant In Press; DOI: 10.12659/AOT.938239  

12 April 2022 : Letter/Correspondence  

Pancreas Allograft Thrombosis as a Post-COVID-19 Complication in a Diabetic Patient After Pancreas Transpla...

Ann Transplant 2022; 27:e935863

In Press

28 Nov 2022 : Original article  

Hepatic Ischemia/Reperfusion Injury After Liver Transplantation Is Not Associated with Early Impairment of ...

Ann Transplant In Press; DOI: 10.12659/AOT.938105  

28 Nov 2022 : Original article  

Modification of Venous Outflow to Avoid Thrombotic Graft Failure in Pancreas Transplantation

Ann Transplant In Press; DOI: 10.12659/AOT.937514  

15 Nov 2022 : Original article  

Changes in Physical Activity Due to Fear of COVID-19 and Its Impact on Depression Among Post-Liver Transpla...

Ann Transplant In Press; DOI: 10.12659/AOT.938239  

14 Nov 2022 : Original article  

Pure Laparoscopic Versus Open Right Hepatectomy in Living Liver Donors: Graft Weight Discrepancy

Ann Transplant In Press; DOI: 10.12659/AOT.938274  

Most Viewed Current Articles

26 Jan 2022 : Review article  

Recurrence of Hepatocellular Carcinoma After Liver Transplantation: Risk Factors and Predictive Models

DOI :10.12659/AOT.934924

Ann Transplant 2022; 27:e934924

24 Jul 2020 : Review article  

Kidney Transplantation in the Times of COVID-19 – A Literature Review

DOI :10.12659/AOT.925755

Ann Transplant 2020; 25:e925755

29 Dec 2021 : Original article  

Efficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...

DOI :10.12659/AOT.933588

Ann Transplant 2021; 26:e933588

24 Aug 2021 : Review article  

Normothermic Machine Perfusion (NMP) of the Liver – Current Status and Future Perspectives

DOI :10.12659/AOT.931664

Ann Transplant 2021; 26:e931664

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358