23 June 2020: Original Paper
Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation
Weiguo Sui 1A* , Qing Gan 1CE* , Fuhua Liu 1B , Minglin Ou 1D , Bingguo Wang 1F , Songbai Liao 1F , Liusheng Lai 1F , Huaizhou Chen 1D , Ming Yang 1G* , Yong Dai 2AG*DOI: 10.12659/AOT.921844
Ann Transplant 2020; 25:e921844
Table 2 Potential metabolite makers of negative ions from Pre vs. NC.
| Metabolite name | m/z | Change | VIP | Fold change | q-value | Related pathway |
|---|---|---|---|---|---|---|
| Threonic acid | 135.0296 | ↑ | 2.920519 | 6.722945 | 0.002249 | Ascorbate and aldarate metabolism |
| Threonic acid | 135.0299 | ↑ | 3.101811 | 8.680666 | 0.002504 | Ascorbate and aldarate metabolism |
| Phosphoribosyl formamidocarboxamide | 365.0538 | ↑ | 1.429341 | 1.667861 | 0.015919 | Purine metabolism; Metabolic pathways; Biosynthesis of secondary metabolites |
| 8(R)-Hydroperoxylinoleic acid | 311.2199 | ↑ | 1.985069 | 3.354787 | 0.049389 | Linoleic acid metabolism |
| 13-L-Hydroperoxylinoleic acid | 311.2199 | ↑ | 1.985069 | 3.354787 | 0.049389 | Linoleic acid metabolism; Metabolic pathways |
| 9(S)-HPODE | 311.2199 | ↑ | 1.985069 | 3.354787 | 0.049389 | Linoleic acid metabolism; Metabolic pathways |
| Taurocholic acid | 514.2828 | ↑ | 3.725514 | 121.6349 | 0.025012 | Primary bile acid biosynthesis; Secondary bile acid biosynthesis; Taurine and hypotaurine metabolism; Metabolic pathways; Bile secretion |
| 8-HETE | 319.2261 | ↓ | 3.539139 | 0.104499 | 0.007557 | Arachidonic acid metabolism; Metabolic pathways; PPAR signaling pathway |
| 8,9-Epoxyeicosatrienoic acid | 319.2261 | ↓ | 3.539139 | 0.104499 | 0.007557 | Arachidonic acid metabolism; Metabolic pathways; Vascular smooth muscle contraction |
| 19(S)-HETE | 319.2261 | ↓ | 3.539139 | 0.104499 | 0.007557 | Arachidonic acid metabolism; Metabolic pathways |
| 11(R)-HETE | 319.2261 | ↓ | 3.539139 | 0.104499 | 0.007557 | Arachidonic acid metabolism |
| 15(S)-HETE | 319.2261 | ↓ | 3.539139 | 0.104499 | 0.007557 | Arachidonic acid metabolism |
| 20-Hydroxyeicosatetraenoic acid | 319.2261 | ↓ | 3.539139 | 0.104499 | 0.007557 | Arachidonic acid metabolism; Metabolic pathways; Vascular smooth muscle contraction |
| 5,6-Epoxy-8,11,14-eicosatrienoic acid | 319.2261 | ↓ | 3.539139 | 0.104499 | 0.007557 | Arachidonic acid metabolism; Metabolic pathways; Vascular smooth muscle contraction |
| 16(R)-HETE | 319.2261 | ↓ | 3.539139 | 0.104499 | 0.007557 | Arachidonic acid metabolism |
| 14,15-Epoxy-5,8,11-eicosatrienoic acid | 319.2261 | ↓ | 3.539139 | 0.104499 | 0.007557 | Arachidonic acid metabolism; Metabolic pathways; Vascular smooth muscle contraction |
| 11,12-Epoxyeicosatrienoic acid | 319.2261 | ↓ | 3.539139 | 0.104499 | 0.007557 | Arachidonic acid metabolism; Metabolic pathways; Vascular smooth muscle contraction |
| 5-HETE | 319.2261 | ↓ | 3.539139 | 0.104499 | 0.007557 | Arachidonic acid metabolism |
| Docosapentaenoic acid | 329.2469 | ↓ | 1.976434 | 0.413162 | 0.008974 | Biosynthesis of unsaturated fatty acids |
| Arachidonic acid | 303.2320 | ↓ | 2.263972 | 0.331697 | 0.000831 | Arachidonic acid metabolism; Linoleic acid metabolism; Biosynthesis of unsaturated fatty acids; Metabolic pathways; Vascular smooth muscle contraction; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Long-term depression; Phototransduction – fly; GnRH signaling pathway; Leishmaniasis; Amoebiasis |
| Taurochenodeoxycholic acid | 498.2879 | ↑ | 2.958113 | 33.07833 | 0.044239 | Primary bile acid biosynthesis; Secondary bile acid biosynthesis; Bile secretion |
| 9,10-Epoxyoctadecenoic acid | 295.2257 | ↓ | 1.591986 | 0.55364 | 0.02713 | Linoleic acid metabolism; Metabolic pathways |
| Alpha-dimorphecolic acid | 295.2257 | ↓ | 1.591986 | 0.55364 | 0.02713 | Linoleic acid metabolism; PPAR signaling pathway |
| 12,13-EpOME | 295.2257 | ↓ | 1.591986 | 0.55364 | 0.02713 | Linoleic acid metabolism; Metabolic pathways |
| 13S-hydroxyoctadecadienoic acid | 295.2257 | ↓ | 1.591986 | 0.55364 | 0.02713 | Linoleic acid metabolism; PPAR signaling pathway |
| Chenodeoxycholic acid glycine conjugate | 448.3056 | ↑ | 2.59988 | 6.271501 | 0.010639 | Primary bile acid biosynthesis; Secondary bile acid biosynthesis; Bile secretion |
| Glycocholic acid | 464.3005 | ↑ | 2.925803 | 9.113965 | 0.015133 | Primary bile acid biosynthesis; Secondary bile acid biosynthesis; Metabolic pathways; Bile secretion |
| 9,12,13-TriHOME | 329.2304 | ↑ | 2.3531 | 3.288932 | 0.006036 | Linoleic acid metabolism |
| 9,10,13-TriHOME | 329.2304 | ↑ | 2.3531 | 3.288932 | 0.006036 | Linoleic acid metabolism |
| Adipate semialdehyde | 129.0544 | ↓ | 1.13189 | 0.720488 | 0.025688 | Caprolactam degradation; Metabolic pathways; Microbial metabolism in diverse environments |
| Ketoleucine | 129.0544 | ↓ | 1.13189 | 0.720488 | 0.025688 | Valine, leucine and isoleucine degradation; Valine, leucine and isoleucine biosynthesis; Glucosinolate biosynthesis; Metabolic pathways; Biosynthesis of secondary metabolites |
| Phosphoribosyl formamidocarboxamide | 401.0251 | ↑ | 1.649845 | 1.751775 | 0.003328 | Purine metabolism; Metabolic pathways; Biosynthesis of secondary metabolites |
| Trans-Cinnamic acid | 147.0438 | ↑ | 1.2355 | 1.388167 | 0.001396 | Phenylalanine metabolism; Phenylpropanoid biosynthesis; Metabolic pathways; Biosynthesis of secondary metabolites; Microbial metabolism in diverse environments |
| The markers were chosen on the basis of VIP from the OPLS-DA greater than 1.0, q-value | ||||||