04 September 2020: Original Paper
Transplantation of a Novel Recombinant Adeno-Associated Virus (pAAV-HE1B19K-TE1A) Demonstrates Higher Anti-Tumor Effects in Tumor Cells
Zhuo-Qing Li 1BCDEF* , Hong Shen 2BCDE* , Kun-Xian Shu 3BCDF , Jian-Jun Li 1BCDF , Yin Tang 4BCD , Jing-Jing Su 4BCF , Jun Yan 1BCD , Jie Yang 3BCF , Ze-Qing Wang 1BCF , Yan Qiu 4BC , Yong Yang 1CF , Yang Liu 4CF , Yong Zhou 5BDEG*DOI: 10.12659/AOT.925013
Ann Transplant 2020; 25:e925013
Table 1 Primers for the PCR assay.
| Gene | Sequences | |
|---|---|---|
| E1B19K | Forward | 5′-GAT AAA TGG AGC GAA GAA ACC C-3′ |
| Reverse | 5′-CGG ACG GAA GAC AAC AGT AGC-3′ | |
| Caspase 3 | Forward | 5′-AGC ACT GGA ATG ACA TCT CGG T-3′ |
| Reverse | 5′-ATG GCT CAG AAG CAC ACA AAC A-3′ | |
| Cytochrome C | Forward | 5′-AAA GGG AGG CAA GCA CAA GA-3′ |
| Reverse | 5′-GAT CAT TTT TGT TCC AGG GAT GTA-3′ | |
| HKII | Forward | 5′-GCC TTT CCG TCC CAG CCT TTA GCC-3′ |
| Reverse | 5′-GGA CTC CTG CGC CGG AGT TTC ATG-3′ | |
| GAPDH | Forward | 5′-AGA TCC CTC CAA AAT CAA GTG G-3′ |
| Reverse | 5′-GGC AGA GAT GAT GAC CCT TTT-3′ |