25 February 2023 : Original article
Detecting Donor-Derived DNA by Real-Time PCR in Recipients Suspected of Graft-Versus-Host-Diseases After Liver Transplantation: A Case Series and Literature Review
Changhee Ha
1ABCDEF, Sang Jin Kim 234ABCDEF, Jong Man Kim 2BE, Jae-Won Joh 2BE, Kee-Taek Jang 5BE, Gyu-Seong Choi 2ABCDE, Eun-Suk Kang 1ABCDE*
DOI: 10.12659/AOT.938287
Ann Transplant 2023; 28:e938287
Table 2 Characteristics of patients with graft-versus-host disease after liver transplantation.
| Case No. | Age/sex | Donor type (relationship/ MELD score) | HLA type* | Pre-LT CMV serology | ||
|---|---|---|---|---|---|---|
| R | D | R | D | |||
| 1 | 66/M | 38/F | Living (Daughter/10) | A24, A33 | A33, A33 | R+/D+ |
| B44, B46 | B44, B44 | |||||
| C1, C7 | C7, C7 | |||||
| DR7, DR8 | DR7, DR7 | |||||
| DQ1, DQ2 | DQ2, DQ2 | |||||
| 2 | 49/M | 24/M | Deceased (Unrelated/36) | A2, A2 | A2, A11 | R+/D+ |
| B13, B35 | B51, B62 | |||||
| DR11, DR12 | DR4, DR4 | |||||
| 3 | 69/F | 43/M | Living (Son/13) | A2, A11 | A2, A26 | R+/D+ |
| B35, B39 | B35, B62 | |||||
| DR8, DR8 | DR8, DR15 | |||||
| ** | # | ## | ||||
| 1 | Diarrhea (23) | High dose steroid, TNF-α inhibitor (30) | Yes (32) | 39.68 | KMR016 (D) | |
| Rash (24)Neutropenia (28) | KMR054 (D) | |||||
| 2 | Pancytopenia (29) | Moderate-dose steroid, TNF-α inhibitor (38) | Yes (41) | 78.38 | KMR033 (R) | |
| Diarrhea (33)Rash (34) | KMR037 (D) | |||||
| 3 | Pancytopenia (80) | Moderate-dose steroid only (90) | No | 4.76 | KMR009 (R) | |
| Hematochezia & Diarrhea (84) | KMR011 (R)KMR036 (R)KMR037 (R)KMR028 (D)KMR047 (D)KMR054 (D) | |||||
| * HLA typing was performed with intermediate resolution sequence-specific oligonucleotide probe hybridization (One Lambda LABType™, Thermo Fisher Scientific Inc., Waltham, MA, USA); ** The first day of GVHD feature appearance after LT; # The first day of GVHD treatment after LT; ## Mean fraction of donor-derived DNA representing donor-cell fraction among recipient cells in peripheral blood. GVHD – graft-versus-host-disease; LT – liver transplantation; R – recipient; D – donor; MELD – Model-for-End-Stage Liver Disease; HLA – human leukocyte antigen; CMV – cytomegalovirus; ddDNA – donor-derived deoxyribonucleic acid; F – female; M – male; TNF-α – tumor necrosis factor α. | ||||||