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25 February 2023: Original Paper

Detecting Donor-Derived DNA by Real-Time PCR in Recipients Suspected of Graft-Versus-Host-Diseases After Liver Transplantation: A Case Series and Literature Review

Changhee Ha 1ABCDEF* , Sang Jin Kim 234ABCDEF* , Jong Man Kim 2BE , Jae-Won Joh 2BE , Kee-Taek Jang 5BE , Gyu-Seong Choi 2ABCDE* , Eun-Suk Kang 1ABCDE*

DOI: 10.12659/AOT.938287

Ann Transplant 2023; 28:e938287

Table 3 Overview of previous chimerism studies of graft-versus-host disease after liver transplantation.

CountryNo. of casesDonor typeUnderlying diseaseMethod
Our study3Living#1: HCC/LCINDEL-qPCR
Deceased#2: Alcoholic LC
Living#3: HCC, LC, HBV
China (2021) []36 1DeceasedHCC, HBVSTR-PCR
Korea (2019) []37 1LivingLC, HBVSTR-PCR
Japan (2019) []21 1LivingPSCFISH
Brazil (2011) []38 1DeceasedHCC, HCVFCM
Japan (2010) []39 1LivingLC, HCVSTR-PCR
France (2009) []20 1DeceasedHCC, LC, HBVSTR-PCR
USA (2009) []40 1LivingPBCFISH
Australia (2009) []6 1DeceasedHBVFISH
Korea (2007) []41 1LivingHCC, LC, HBVSTR-PCR
USA (2007) []42 3Deceased#1: PSC#2: PSC#3: HCC, HBVSTR-PCR
USA (2006) []27 1DeceasedPSC, HCCFISH
USA (2005) []43 1DeceasedAlcoholic LCSTR-PCR
Our studyPB#1: 39.68%30No
#2: 78.38%38No
#3: 4.76%90Yes
China (2021) []36 PB4.82%31Yes
Korea (2019) []37 PB62.4%39No
Japan (2019) []21 PB34%48Yes
Brazil (2011) []38 PB3~4%32No
Japan (2010) []39 PB> 95%Within 35No
France (2009) []20 PB8.9%Within 21Yes
USA (2009) []40 PB/BM89.4% (PB)/79.4% (BM)28No
Australia (2009) []6 BM18%21Yes
Korea (2007) []41 PB10%115No
USA (2007) []42 PB#1: 7%70Yes
#2: 20%7Yes
#3: 26.5%14No
USA (2006) []27 PB16%82No
USA (2005) []43 PB/BM96% (PB)/30% (BM)240No
* Chimerism test day after transplantation.
HCC – hepatocellular carcinoma; LC – liver cirrhosis; HBV – hepatitis B virus infection; HCV – hepatitis C virus infection; PBC – primary biliary cirrhosis; PSC – primary sclerosing cholangitis; INDEL-qPCR – real-time quantitative polymerase chain reaction targeting insertions and/or deletions of chromosomes; STR-PCR – polymerase chain reaction targeting short tandem repeat; FISH – fluorescence in situ hybridization; FCM – flow cytometry; PB – peripheral blood; BM – bone marrow.

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Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358