25 February 2023 : Original article
Detecting Donor-Derived DNA by Real-Time PCR in Recipients Suspected of Graft-Versus-Host-Diseases After Liver Transplantation: A Case Series and Literature Review
Changhee Ha
1ABCDEF, Sang Jin Kim 234ABCDEF, Jong Man Kim 2BE, Jae-Won Joh 2BE, Kee-Taek Jang 5BE, Gyu-Seong Choi 2ABCDE, Eun-Suk Kang 1ABCDE*
DOI: 10.12659/AOT.938287
Ann Transplant 2023; 28:e938287
Table 3 Overview of previous chimerism studies of graft-versus-host disease after liver transplantation.
| Country | No. of cases | Donor type | Underlying disease | Method |
|---|---|---|---|---|
| Our study | 3 | Living | #1: HCC/LC | INDEL-qPCR |
| Deceased | #2: Alcoholic LC | |||
| Living | #3: HCC, LC, HBV | |||
| China (2021) []36 | 1 | Deceased | HCC, HBV | STR-PCR |
| Korea (2019) []37 | 1 | Living | LC, HBV | STR-PCR |
| Japan (2019) []21 | 1 | Living | PSC | FISH |
| Brazil (2011) []38 | 1 | Deceased | HCC, HCV | FCM |
| Japan (2010) []39 | 1 | Living | LC, HCV | STR-PCR |
| France (2009) []20 | 1 | Deceased | HCC, LC, HBV | STR-PCR |
| USA (2009) []40 | 1 | Living | PBC | FISH |
| Australia (2009) []6 | 1 | Deceased | HBV | FISH |
| Korea (2007) []41 | 1 | Living | HCC, LC, HBV | STR-PCR |
| USA (2007) []42 | 3 | Deceased | #1: PSC#2: PSC#3: HCC, HBV | STR-PCR |
| USA (2006) []27 | 1 | Deceased | PSC, HCC | FISH |
| USA (2005) []43 | 1 | Deceased | Alcoholic LC | STR-PCR |
| * | ||||
| Our study | PB | #1: 39.68% | 30 | No |
| #2: 78.38% | 38 | No | ||
| #3: 4.76% | 90 | Yes | ||
| China (2021) []36 | PB | 4.82% | 31 | Yes |
| Korea (2019) []37 | PB | 62.4% | 39 | No |
| Japan (2019) []21 | PB | 34% | 48 | Yes |
| Brazil (2011) []38 | PB | 3~4% | 32 | No |
| Japan (2010) []39 | PB | > 95% | Within 35 | No |
| France (2009) []20 | PB | 8.9% | Within 21 | Yes |
| USA (2009) []40 | PB/BM | 89.4% (PB)/79.4% (BM) | 28 | No |
| Australia (2009) []6 | BM | 18% | 21 | Yes |
| Korea (2007) []41 | PB | 10% | 115 | No |
| USA (2007) []42 | PB | #1: 7% | 70 | Yes |
| #2: 20% | 7 | Yes | ||
| #3: 26.5% | 14 | No | ||
| USA (2006) []27 | PB | 16% | 82 | No |
| USA (2005) []43 | PB/BM | 96% (PB)/30% (BM) | 240 | No |
| * Chimerism test day after transplantation. HCC – hepatocellular carcinoma; LC – liver cirrhosis; HBV – hepatitis B virus infection; HCV – hepatitis C virus infection; PBC – primary biliary cirrhosis; PSC – primary sclerosing cholangitis; INDEL-qPCR – real-time quantitative polymerase chain reaction targeting insertions and/or deletions of chromosomes; STR-PCR – polymerase chain reaction targeting short tandem repeat; FISH – fluorescence in situ hybridization; FCM – flow cytometry; PB – peripheral blood; BM – bone marrow. | ||||