08 January 2021>: Original Paper
Amniotic Fluid Transplantation Alleviates Hematopoietic Deficits in Experimental Rat Aplastic Anemia
Libin Liang 1BC , Xiaojun Wang 1B , Mingming Li 1B , Luyang Qin 2B , Xinglong Tong 1ACDEF*DOI: 10.12659/AOT.928047
Ann Transplant 2021; 26:e928047
Figure 2 AFSCs in vitro expansion in pure amniotic fluid. (A) AFSCs in freshly-collected amniotic fluid (40×). (B) AFSCs in vitro cultured in pure amniotic fluid for 30 min (40×). (C) AFSCs cultured for 48 h in DMEM medium (20% serum) (40×). (D) Ultrastructure of AFSCs in freshly-collected amniotic fluid. (E) Ultrastructure of AFSCs after cultured in pure amniotic fluid. (F) Autophagosomes were observed in the cytoplasm when the expanded cells covered over 80% of the culture surface.