08 January 2021 : Original article
Amniotic Fluid Transplantation Alleviates Hematopoietic Deficits in Experimental Rat Aplastic Anemia
Libin Liang1BC, Xiaojun Wang1B, Mingming Li1B, Luyang Qin2B, Xinglong Tong1ACDEF*DOI: 10.12659/AOT.928047
Ann Transplant 2021; 26:e928047
Figure 2 AFSCs in vitro expansion in pure amniotic fluid. (A) AFSCs in freshly-collected amniotic fluid (40×). (B) AFSCs in vitro cultured in pure amniotic fluid for 30 min (40×). (C) AFSCs cultured for 48 h in DMEM medium (20% serum) (40×). (D) Ultrastructure of AFSCs in freshly-collected amniotic fluid. (E) Ultrastructure of AFSCs after cultured in pure amniotic fluid. (F) Autophagosomes were observed in the cytoplasm when the expanded cells covered over 80% of the culture surface.