Figure 3 Effect of apraglutide on leukopenia in BALB/c mice. Effect on polymorphonuclear cell count following chemotherapy (A) and effect on the myelosuppressive activity of cytarabine (B). (A) Treatment group mean±SEM are shown (n=6 per treatment group); cell counts were determined in specifically designated animals. (B) Immunosuppressive activity was monitored at the end of the study. Samples were collected at euthanasia (n=3). Data are shown as mean±SEM values. As determined by the unpaired t-test according to the Holm-Sidak method for multiple comparisons, the mean number of WBC, NEUT, and LYMPH was significantly decreased in animals treated with cytarabine alone or in combination with apraglutide, compared with the control (* P<0.05, # P<0.01) and recovery groups (& P<0.05, $ P<0.01). No difference was observed between animals treated with cytarabine alone or in combination with apraglutide. No difference was observed either between the control mice and those in the recovery group treated with cytarabine plus apraglutide (NS).