Circulating NKG2A–NKG2D+ CD56dimCD16+ Natural Killer (NK) Cells as Mediators of Functional Immunosurveillance in Kidney Transplant Recipients

Li Zhu; Hristos Karakizlis; Rolf Weimer; Christian Morath; Naruemol Ekpoom; Eman H. Ibrahim; Gerhard Opelz; Volker Daniel

doi:10.12659/AOT.925162

22 December 2020 : Original article

Circulating NKG2A–NKG2D+ CD56dimCD16+ Natural Killer (NK) Cells as Mediators of Functional Immunosurveillance in Kidney Transplant Recipients

Li Zhu^12ABCDEF, Hristos Karakizlis^3B, Rolf Weimer^3B, Christian Morath^4B, Naruemol Ekpoom^2B, Eman H. Ibrahim^2BE, Gerhard Opelz^2ADEF, Volker Daniel^2ABCDEF*

DOI: 10.12659/AOT.925162

Ann Transplant 2020; 25:e925162

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Table 2 Antibody panel for flow cytometric tests of peripheral blood lymphocytes.

	FITC	PE	PE-Cy7	APC
		IgG		IgG
		CD158a		CD158e
		NKG2D		NKG2A
		IL-4R		CD158b
		CD69		CD107a
		CD119		CD25
		TGFβRII		IL-10R
		cIL-10	NKG2A	cINFγ
		cIL-4	NKG2A	cTGFβ
		cPerforin	NKG2A	cGranzymeB
		cIL17	CD4	CD8
		Foxp3	CD127	CD25
	cINFγ	Foxp3	CD127	CD25
Table shows the monoclonal antibody panel with the corresponding fluorochromes. Monoclonal antibodies in italic font were used in every tube whereas monoclonal antibodies written in regular font were only used in certain tubes.

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Circulating NKG2A–NKG2D+ CD56dimCD16+ Natural Killer (NK) Cells as Mediators of Functional Immunosurveillance in Kidney Transplant Recipients

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