01 December 2021 : Original article
Solid-Phase C1q/C3d Fixing Readouts Correlate with High Median Fluorescence Intensity (MFI) De Novo Donor-Specific HLA Antibodies and C4d⁺ Antibody-Mediated Rejection in Kidney Transplant Recipients
Vasishta S. Tatapudi1BDEF, Dessislava Kopchaliiska2ABC, Gilberto J. da Gente2C, Owen F. Buenaventura2BC, Manpreet Singh3BC, Zoltan Laszik4BC, Deborah B. Adey1CDE, Raja Rajalingam
2ADEF*
DOI: 10.12659/AOT.934175
Ann Transplant 2021; 26:e934175
Table 3 Kidney transplant recipients with de novo donor-specific HLA antibodies (dnDSA) of IgG MFI ≥5000 but negative for C3d and C1q-binding. The median fluorescence intensity (MFI) of each dnDSA is given in parenthesis.
| Recipient | Sex | Re-Tx | # DSA | Highest MFI dnDSA | Additional dnDSA (MFI) | C4d | Rejection |
|---|---|---|---|---|---|---|---|
| 1 | F | No | 2 | DQ5 (11056) | DQ2 (6345) | + | cAMR |
| 2 | M | No | 1 | DQ6 (8784) | − | cAMR | |
| 3 | M | Yes | 3 | B35 (8576) | B7 (6429), DR11 (1317) | + | aAMR |
| 4 | M | Yes | 1 | DQ7 (8444) | − | ACR | |
| 5 | F | No | 2 | DQ7 (8357) | DQ8 (7273) | + | aAMR |
| 6 | F | No | 1 | DQ7 (7851) | − | No | |
| 7 | F | No | 1 | DQ4 (7706) | − | No | |
| 8 | F | Yes | 3 | DR4 (7407) | B55 (2334), DQ8 (3374) | − | ACR |
| 9 | M | No | 1 | DQ7 (7096) | − | cAMR | |
| 10 | M | No | 5 | B39 (6383) | A24 (1973), B35 (2742), Cw4 (5271), Cw7 (2562) | − | ACR |
| 11 | M | No | 1 | B44 (5971) | − | No | |
| 12 | M | No | 5 | A2 (5277) | A3 (2462), B48 (5209), DR8 (1428), DR11 (1360) | + | aAMR |
| 13 | F | No | 4 | A2 (5218) | B8 (5161), B60 (1683), Cw3 (2105) | + | cAMR |
| 14 | M | No | 1 | DQ7 (5188) | − | No | |
| 15 | M | No | 2 | DQ5 (5089) | DR14 (3759) | − | ACR |
| 16 | M | No | 2 | DQ4 (5058) | DR53 (1931) | − | No |