05 July 2022 : Original article
Cardiac and Pulmonary Histopathology in Baboons Following Genetically-Engineered Pig Orthotopic Heart Transplantation
Silvio H. Litovsky1BDE, Jeremy B. Foote2BCDE, Abhijit Jagdale3BE, Gregory Walcott4BE, Hayato Iwase3BDE, Mohamed H. Bikhet3BDE, Takayuki Yamamoto3BE, Christophe Hansen-Estruch3BE, Mohamed B. Ezzelarab5BE, David Ayares6AE, Waldemar F. Carlo7BE, Leslie A. Rhodes7BE, Jack H. Crawford8BE, Santiago Borasino8BE, Robert J. Dabal8BE, Luz A. Padilla8BE, Hidetaka Hara3BE, David K.C. Cooper3ABDEFG*, David C. Cleveland8ABDEFGDOI: 10.12659/AOT.935338
Ann Transplant 2022; 27:e935338
Table 1 Experimental details of pig orthotopic heart transplants in baboons (n=4).
| Baboon (weight) | Pig genotype (weight) | Cold ischemic time (mins) | Survival | Clinical course | Cause of death |
|---|---|---|---|---|---|
| B217 (8.5 kg) | GTKO. hCD55 (13.0 kg) | 130(a) | 3 hours | Ventricular arrhythmiasVentricular fibrillation | Cardiac arrest |
| B15416 (9.5 kg) | GTKO.hCD55 (12.1 kg) | 63(b) | 3 months | Sinus tachycardia (days 0–30)Hypoxia (days 0–7)Hypertension (days 0–30)Central line infection( – day 14)Left ventricular hypertrophy (day 30) | Sudden death – dysrhythmia suspected |
| B 1917 (8.3 kg) | GTKO.hCD46. hTBM (9.9 kg) | 77(b) | 8 months | Hypoxia (days 0–7)Hypertension (days 0–90)Tachycardia (days 0–90)Increased troponin I (day 180)Sudden death (day 241) | AMR and CMR |
| B37367 (6.0 kg) | GTKO.hCD46. hTBM (6.0 kg) | 71(b) | 4 hours | Hypoxia, Tachypnea, Tachycardia, Hypertension followed by hypotension, Ventricular fibrillation with resuscitation | Idioventricular rhythm, cardiac arrest |
| (a) Stored in cold University of Wisconsin (UW) solution in ice for 60 minutes before OHTx. (b) Perfused with cold del Nido solution, and immediately transplanted. No ice storage. GTKO – α1,3-galactosyltransferase gene-knockout; hCD46 – human complement-regulatory protein CD46; hCD55 – human complement-regulatory protein CD55; hTBM – human thrombomodulin; AMR – antibody-mediated rejection; CMR – cell-mediated rejection. | |||||