05 July 2022>: Original Paper
Cardiac and Pulmonary Histopathology in Baboons Following Genetically-Engineered Pig Orthotopic Heart Transplantation
Silvio H. Litovsky 1BDE , Jeremy B. Foote 2BCDE , Abhijit Jagdale 3BE , Gregory Walcott 4BE , Hayato Iwase 3BDE , Mohamed H. Bikhet 3BDE , Takayuki Yamamoto 3BE , Christophe Hansen-Estruch 3BE , Mohamed B. Ezzelarab 5BE , David Ayares 6AE , Waldemar F. Carlo 7BE , Leslie A. Rhodes 7BE , Jack H. Crawford 8BE , Santiago Borasino 8BE , Robert J. Dabal 8BE , Luz A. Padilla 8BE , Hidetaka Hara 3BE , David K.C. Cooper 3ABDEFG* , David C. Cleveland 8ABDEFGDOI: 10.12659/AOT.935338
Ann Transplant 2022; 27:e935338
Table 1 Experimental details of pig orthotopic heart transplants in baboons (n=4).
Baboon (weight) | Pig genotype (weight) | Cold ischemic time (mins) | Survival | Clinical course | Cause of death |
---|---|---|---|---|---|
B217 (8.5 kg) | GTKO. hCD55 (13.0 kg) | 130(a) | 3 hours | Ventricular arrhythmiasVentricular fibrillation | Cardiac arrest |
B15416 (9.5 kg) | GTKO.hCD55 (12.1 kg) | 63(b) | 3 months | Sinus tachycardia (days 0–30)Hypoxia (days 0–7)Hypertension (days 0–30)Central line infection( – day 14)Left ventricular hypertrophy (day 30) | Sudden death – dysrhythmia suspected |
B 1917 (8.3 kg) | GTKO.hCD46. hTBM (9.9 kg) | 77(b) | 8 months | Hypoxia (days 0–7)Hypertension (days 0–90)Tachycardia (days 0–90)Increased troponin I (day 180)Sudden death (day 241) | AMR and CMR |
B37367 (6.0 kg) | GTKO.hCD46. hTBM (6.0 kg) | 71(b) | 4 hours | Hypoxia, Tachypnea, Tachycardia, Hypertension followed by hypotension, Ventricular fibrillation with resuscitation | Idioventricular rhythm, cardiac arrest |
(a) Stored in cold University of Wisconsin (UW) solution in ice for 60 minutes before OHTx. (b) Perfused with cold del Nido solution, and immediately transplanted. No ice storage. GTKO – α1,3-galactosyltransferase gene-knockout; hCD46 – human complement-regulatory protein CD46; hCD55 – human complement-regulatory protein CD55; hTBM – human thrombomodulin; AMR – antibody-mediated rejection; CMR – cell-mediated rejection. |