15 January 2004 : Original article
The Biocompatibility of Nitrided Titanium Alloy for Bone Implantation Studied by Laser Flow Cytometry, Laser Scanning Cytometry and Confocal Microscopy
E Czarnowska, A Sowinska, B Cukrowska, M Godlewski, T WierzchonAnn Transplant 2004; 9(1A): 72-75 :: ID: 15639
Abstract
Objectives: Cell adhesion is a fundamental process influencing cell growth and dispersal, and can be studied by different methods.
Methods: The aim of our study was to evaluate the ability of human fibroblasts and osteoblasts to adhere to samples with nitrided surface layers produced under glow discharge conditions on titanium alloy using laser flow cytometry (FC), laser scanning cytometry (LSC) and confocal microscopy (CM). The expression of fibronectin and its receptor – α5β1 integrin (the CD49e molecule) was analyzed.
Results and Conclusions: Our results showed that fibroblasts and osteoblast-like cells (Saos-2 cell lines) adhere to a TiN+Ti2N+αTi(N) surface layer, which favours its application in bone surgery. Employment of LSC together with CM for analysis of cell behavior in contact with biomaterials seems to be very useful, while FC analysis of chemically harvested cells should be carefully taken into account in the study of cell adhesion processes.
Keywords: Nitrided Titanium Alloy, Cell Adhesion, fibronectin, CD49e, Laser Scanning
In Press
Original article
Diagnostic Utility of FAR1 Methylation Levels in Hepatocellular Carcinoma Patients Undergoing Liver Transpl...Ann Transplant In Press; DOI: 10.12659/AOT.951568
Original article
Inferior Long-Term Outcome of Fatty Liver Allografts After Orthotopic Liver TransplantationAnn Transplant In Press; DOI: 10.12659/AOT.950589
Database Analysis
Identification and Validation of Liver Transplantation-Induced Acute Lung Injury Biomarkers Using a Bioinfo...Ann Transplant In Press; DOI: 10.12659/AOT.950289
Original article
Survival and Recurrence in Liver Transplant Patients With Intrahepatic Cholangiocarcinoma and Hepatocellula...Ann Transplant In Press; DOI: 10.12659/AOT.950997
Most Viewed Current Articles
24 Aug 2021 : Review article 18,372
Normothermic Machine Perfusion (NMP) of the Liver – Current Status and Future PerspectivesDOI :10.12659/AOT.931664
Ann Transplant 2021; 26:e931664
05 Apr 2022 : Original article 14,731
Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver TransplantationDOI :10.12659/AOT.935604
Ann Transplant 2022; 27:e935604
22 Nov 2022 : Original article 14,244
Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...DOI :10.12659/AOT.937988
Ann Transplant 2022; 27:e937988
29 Dec 2021 : Original article 13,752
Efficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...DOI :10.12659/AOT.933588
Ann Transplant 2021; 26:e933588