Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

15 December 2004

Influence of Bacterial Antigens On Activation of Human Splenic Dendritic Cells

J Stanislawska, B Interewicz, W L Olszewski

Ann Transplant 2004; 9(4): 54-58 :: ID: 13034

Abstract

The dendritic cells (DC) play crucial role in initiation and modulation of immune response especially innate immune response. Toll like receptors (TLR) on DC are receptors involved in innate immunity and recognizing conserved bacterial antigens like LPS and bacterial DNA. TLRs can also respond to some endogenous ligands (heat-shock proteins, heparan sulfate, fibrinogen and the contest of necrotic cells). Recognition of such endogenous substances would be a critical step in response to viruses, tumors and possibly to transplants. We investigated the influence bacterial antigens on splenic cell population enriched in DCs. After incubation with bacterial antigens the percentage of DC expressing HLA-DR+ and CD123+ cells increased whereas that of CD68+ and CD14+ decreased. In the untreated population of human splenic DC minimal expression of TLR2, TLR3 and CD123 was found, while other receptors were not detected. After incubation with bacteria a marked increase of CD83, TLR2, TLR3 and TLR4 was observed. Treatment with LPS increased expression of TLR2, TLR4, Hsp60 and Hsp90. Stimulation by bacterial DNA resulted mainly in Hsp60 and TLR9 expression. These observation may throw light on the mechanism of exacerbation of the rejection of transplanted organs by microbial stimulation.

Keywords: Bacterial Antigens, innate immunity, Tlrs

Add Comment 0 Comments

Most Viewed Current Articles

15 Aug 2023 : Review article   7,203

Free-Circulating Nucleic Acids as Biomarkers in Patients After Solid Organ Transplantation

DOI :10.12659/AOT.939750

Ann Transplant 2023; 28:e939750

03 Jan 2023 : Original article   7,086

Impact of Autologous Stem Cell Transplantation on Primary Central Nervous System Lymphoma in First-Line and...

DOI :10.12659/AOT.938467

Ann Transplant 2023; 28:e938467

16 May 2023 : Original article   6,914

Breaking Antimicrobial Resistance: High-Dose Amoxicillin with Clavulanic Acid for Urinary Tract Infections ...

DOI :10.12659/AOT.939258

Ann Transplant 2023; 28:e939258

28 May 2024 : Original article   6,394

Effect of Dexmedetomidine Combined with Remifentanil on Emergence Agitation During Awakening from Sevoflura...

DOI :10.12659/AOT.943281

Ann Transplant 2024; 29:e943281

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358