Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

15 January 2004 : Original article  

Human Omentum Majus as A Potential Source of Osteogenic Cells for Tissue Engineering (preliminary report)

P Kowalczyk, R M Olkowski, E Sienkiewicz-Latka, W Lisik, M Sinski, M Kosieradzki, Z Wierzbicki, J Przybylski, M Lewandowska-Szumiel

Ann Transplant 2004; 9(1A): 61-63 :: ID: 15636

Abstract

For the skeletal tissue engineering autologous stem cells are obtained from the bone marrow (BM). However, BM procurement procedures may be painful and may yield low number of stem cells upon processing. There is a need for other convenient stem cells sources.
The omentum majus is potential source of mesenchymal tissues. Thus the aim of this study was to determine if stem cells potentially present in human omentum majus could differentiate into osteogenic tissue. The observations were also performed in the presence of chosen biomaterials (steel, titanium, alumina, hydroxyapatite).
Collected cells were incubated on 96 wells plate (15000 cells per well) per one week in control media (i.e. DMEM + 10% FBS) without vitamin C and with vitamin C, and media enriched with: vitamin D3, dexamethasone. Human osteoblasts were used as a control. Osteocalcin level and alkaline phosphatase activity were compared between groups. Our results suggest that in humans, the omentum majus adipose tissue is able to respond to vitamin C, vitamin D3 and dexamethasone in a manner similar to osteoblasts. The results were confirmed on several chosen biomaterials. It suggests that these cells may express an osteoblast-like phenotype. Thus omentum may be taken into account as potential source of stem cells for skeletal reconstruction.

Keywords: Tissue Engineering, Mesenchymal Stem Cells, Omentum Majus, Osteoblasts

Add Comment 0 Comments

In Press

02 Apr 2024 : Original article  

Liver Transplantation from Brain-Dead Donors with Hepatitis B or C in South Korea: A 2014-2020 Korean Organ...

Ann Transplant In Press; DOI: 10.12659/AOT.943588  

02 Apr 2024 : Original article  

Effect of Dexmedetomidine Combined with Remifentanil on Emergence Agitation During Awakening from Sevoflura...

Ann Transplant In Press; DOI: 10.12659/AOT.943281  

03 Apr 2024 : Review article  

Alternative Therapies in Transplantology as a Promising Perspective in Medicine

Ann Transplant In Press; DOI: 10.12659/AOT.943387  

15 Apr 2024 : Original article  

Physical Activity Levels in Transplant Recipients

Ann Transplant In Press; DOI: 10.12659/AOT.944101  

Most Viewed Current Articles

05 Apr 2022 : Original article  

Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver Transplantation

DOI :10.12659/AOT.935604

Ann Transplant 2022; 27:e935604

12 Jan 2022 : Original article  

Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...

DOI :10.12659/AOT.934738

Ann Transplant 2022; 27:e934738

22 Nov 2022 : Original article  

Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...

DOI :10.12659/AOT.937988

Ann Transplant 2022; 27:e937988

15 Mar 2022 : Case report  

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358