Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

02 December 2002

Interaction of E.Coli with Dendritic Cells – A Model for Studies of Graft Infections

J Stanislawska, B Interewicz, E Szyper, W L Olszewski

Ann Transplant 2002; 7(4): 42-45 :: ID: 496267

Abstract

The rejection process of skin allografts is mediated by dendritic cells (DC) and lymphocytes. The recipient DCs are engaged not only into an allogenic but also antibacterial reaction to the penetrating bacteria. The capacity of these cells to sample sites of pathogen entry, respond to microbial signals and activate naive T cells suggests a critical role for DC in initiating antimicrobial immunity. In our study, we investigated the ability of the green fluorescent protein (Gfp) labelled E.coli to infect DC. We studied kinetics of in vitro and in vivo adherence and incorporation of E.coli by rat spleen and bone marrow (BM) DC. Bacterial adherence to the cell surface was observed after 2h incubation of DC with bacteria. A 24-hour culture of DC from both sources was followed by bacterial adherence to all cells and engulfment by at least 50% of cells. There was an increased expression of the phenotypic markers on the DC cultured with E.coli. The Gfp-labelled E.coli should be useful for studies of the activation of dendritic cells. The method will allow to study the process of simultaneous activation of DC by allo- and bacterial antigens.

Keywords: E.coli, Endocytosis, Bacterial Adhesion

Add Comment 0 Comments

In Press

06 Feb 2024 : Case report  

Successful Sequential Liver and Isolated Intestine Transplantation for Mitochondrial Neurogastrointestinal ...

Ann Transplant In Press; DOI: 10.12659/AOT.941881  

12 Feb 2024 : Original article  

No Prognostic Impact of Graft-to-Recipient Weight Ratio on Hepatocellular Carcinoma Recurrence Following Li...

Ann Transplant In Press; DOI: 10.12659/AOT.942767  

21 Feb 2024 : Original article  

Use of LCP-Tacrolimus (LCPT) in Kidney Transplantation: A Delphi Consensus Survey of Expert Clinicians

Ann Transplant In Press; DOI: 10.12659/AOT.943498  

Most Viewed Current Articles

05 Apr 2022 : Original article  

Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver Transplantation

DOI :10.12659/AOT.935604

Ann Transplant 2022; 27:e935604

12 Jan 2022 : Original article  

Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...

DOI :10.12659/AOT.934738

Ann Transplant 2022; 27:e934738

22 Nov 2022 : Original article  

Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...

DOI :10.12659/AOT.937988

Ann Transplant 2022; 27:e937988

15 Mar 2022 : Case report  

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358