15 December 2002
Modulation of Human Anti-Pig T cell Responses by Monoclonal Antibodies Directed to Porcine CD45 Molecules
T Bedke, W Baars, R SchwinzerAnn Transplant 2003; 8(3): 35-38 :: ID: 7529
Abstract
Antibody-mediated targeting of pig costimulatory molecules is assumed to be a possible strategy to achieve donor-specific tolerance after xenotransplantation. However, porcine molecules of the B7 family (e.g. CD86) are expressed on typical antigen presenting cells (APC) and also on vascular endothelial cells. Thus, in vascularized porcine xenografts the usage of therapeutic anti-B7 monoclonal antibodies (mAb) might be associated with damage of the endothelium. Objective: In the present study we asked whether modulation of human T cell reactivity can be obtained by targeting molecules selectively expressed on pig leucocytes. Methods: MAb directed to pig CD45 were tested for their capacity to modulate the in vitro activation of human T cells induced by porcine peripheral blood mononuclear cells. Results: Porcine stimulatory cells induced significant proliferation of human T cells. In the presence of porcine CD45 mAb human T cell responses were reduced by 30–40%. The inhibitory effects were most pronounced when CD45RA mAb were used whereas mAb directed to CD45RC isoforms only moderately inhibited human T cell activation. The tested antibodies had no effects on human T cell activation induced by mitogens or by alloantigen. Conclusion: Manipulation of CD45 molecules on pig leucocytes may reduce their potential to stimulate human T cells. In recipients of vascularized porcine xenografts the usage of anti-pig CD45 mAb could be an approach to block the direct pathway of T cell activation initiated by porcine APC without affecting the endothelium of the graft.
SARS-CoV-2/COVID-19
29 March 2023 : Editorial
Editorial: Effective Direct-Acting Antiviral Treatments Support Global and National Programs to Eliminate H...Med Sci Monit In Press; DOI: 10.12659/MSM.940519
06 December 2022 : Original article
Changes in Physical Activity Due to Fear of COVID-19 and Its Impact on Depression Among Post-Liver Transpla...Ann Transplant 2022; 27:e938239
04 October 2022 : Original article
Incidence of COVID-19 and Identification of Possible Risk Factors Associated with COVID-19 in Acute Renal T...Ann Transplant 2022; 27:e937688
12 April 2022 : Letter/Correspondence
Pancreas Allograft Thrombosis as a Post-COVID-19 Complication in a Diabetic Patient After Pancreas Transpla...Ann Transplant 2022; 27:e935863
In Press
27 Mar 2023 : Original article
Outcomes of Kidney Transplantation in Older RecipientsAnn Transplant In Press; DOI: 10.12659/AOT.938692
27 Mar 2023 : Original article
The Incidence of Brain-Dead Donors Based on Screening and Management Led by IntensivistsAnn Transplant In Press; DOI: 10.12659/AOT.939521
22 Mar 2023 : Original article
Effect of Sodium Bicarbonate Ringer’s Solution on Intraoperative Blood Gas Analysis and Postoperative Recov...Ann Transplant In Press; DOI: 10.12659/AOT.939097
17 Mar 2023 : Original article
Converting from Tenofovir Disoproxil Fumarate to Tenofovir Alafenamide in Patients with Hepatitis B Followi...Ann Transplant In Press; DOI: 10.12659/AOT.938731
Most Viewed Current Articles
24 Aug 2021 : Review article
Normothermic Machine Perfusion (NMP) of the Liver – Current Status and Future PerspectivesDOI :10.12659/AOT.931664
Ann Transplant 2021; 26:e931664
26 Jan 2022 : Review article
Recurrence of Hepatocellular Carcinoma After Liver Transplantation: Risk Factors and Predictive ModelsDOI :10.12659/AOT.934924
Ann Transplant 2022; 27:e934924
29 Dec 2021 : Original article
Efficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...DOI :10.12659/AOT.933588
Ann Transplant 2021; 26:e933588
15 Mar 2022 : Case report
Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...DOI :10.12659/AOT.935860
Ann Transplant 2022; 27:e935860