01 December 2007
Inosine monophosphate dehydrogenase (IMPDH) activity after mycophenolate mofetil (MMF) in renal transplant patients receiving different immunosuppressive combined therapy and stable after at least 3 monthsâ€™ administrationL. R. Chiarelli, M. Molinaro, C. Libetta, C. Tinelli, L. Cosmai, G. Valentini, A. Dal Canton, M. Regazzi
Ann Transplant 2008; 13(1): 42-42 :: ID: 880208
Background: Long-term therapy with MMF may induce IMPDH activity in peripheral blood mononuclear cells (MNC), reducing its immunosuppressive properties.
Material/Methods: IMPDH activity was measured in MNCs samples from 73 stable kidney transplant patients (median treatment time: 34 months, range: 3-75), before (t0) and 2 hours after (t2) MMF administration and monitored for up to 12 months. MMF treatment was combined with cyclosporine (CsA group) in 24 subjects, tacrolimus (TAC group) in 33, and sirolimus (SRL group) in 16. Samples from 95 healthy subjects were used as controls.
Results: IMPDH activity in healthy subjects was highly variable, mean 8.33±6.93 nmol/h/mg, with no relevant association with sex or age. In transplant recipients, during 12 months of MMF therapy, t0 IMPDH activity increased from 5.5±3.93 to 11.66±6.45 nmol/h/mg, with considerable intra- and interpatient variability (about 60%), and with no decrease in inhibition level (73.3%, range: 54.0-96.6). No significant intergroup differences were found. Mean concentrations of CsA, TAC, SRL and MPA were 142.4, 9.4, 12.9 ng/ml and 3.38 μg/ml. Four patients experienced acute rejection (AR) during the 12-month follow-up. IMPDH activity at t0 was increased during rejection versus non-rejection in 3 patients. No differences in enzyme inhibition levels were observed.
Conclusions: Despite the association found between IMPDH activity and AR, the high intrapatient variability affects its effectiveness as a predictive tool in long-term transplant outcomes. Further trials, also including pre-transplant data on both IMPDH expression and biological activity, are warranted to better assess the rule of IMPDH activity as biomarker for MPA effect in clinical practice.
Keywords: Mycophenolate Mofetil, combined therapy, Pulsed Doppler tissue imaging Dobutamine stress echocardiography
10 Nov 2023 : Original articleEffects of Preservation of Donor Liver Gastroduodenal Artery on Post-Transplant Biliary Complications in 18...
Ann Transplant In Press; DOI: 10.12659/AOT.941699
07 Nov 2023 : Original articleAllogeneic Hematopoietic Stem Cell Transplantation Can Improve Prognosis of Extramedullary Infiltration Pos...
Ann Transplant In Press; DOI: 10.12659/AOT.942197
06 Nov 2023 : Original articleClinical Outcomes of Administration of Rituximab for Desensitization in Liver Transplant Patients with Pref...
Ann Transplant In Press; DOI: 10.12659/AOT.941456
Most Viewed Current Articles
24 Aug 2021 : Review articleNormothermic Machine Perfusion (NMP) of the Liver – Current Status and Future Perspectives
Ann Transplant 2021; 26:e931664
26 Jan 2022 : Review articleRecurrence of Hepatocellular Carcinoma After Liver Transplantation: Risk Factors and Predictive Models
Ann Transplant 2022; 27:e934924
29 Dec 2021 : Original articleEfficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...
Ann Transplant 2021; 26:e933588