05 December 2008
No evidence for PERV release by islet cells from German landrace pigsMarkus Irgang, Christine Laue, Florian Velten, Reinhard Kurth, Jürgen Schrezenmeier, Joachim Denner
Ann Transplant 2008; 13(4): 59-66 :: ID: 880220
Background: Islet cells from pig could be used as an alternative to the current treatment of diabetic patients. However, xenotransplantation from pig to humans may be associated with the risk of transmission of porcine endogenous retroviruses (PERVs) that are present in the genome of all pigs and infect human cells in vitro. Although transplantation of pig islet cells for treatment of diabetes may be not accompanied by immunosuppression that may facilitate virus survival, since islets will be used encapsulated, it is nevertheless of importance to study whether islet cells release PERVs able to infect human cells during co-incubation.
Material/Methods: Isolated islets from German landrace pigs were incubated with highly susceptible human 293 cells for one week. In order to prevent microchimerism 293 cells were made neomycin-resistant (293[sup]neo+[/sup]), that allows the elimination of pig cells by a selection medium. The infection of 293[sup]neo+ [/sup]target cells was analysed by PCR using PERV-specific primers up to five weeks after co-cultivation. In addition, expression of viral mRNA in pig islet cells was studied by RT-PCR analysis, the expression of viral protein by FACS analysis.
Results: Despite the presence of numerous PERV proviruses in the genome of all pigs, no expression of PERV was observed in German landrace pig islet cells, neither as mRNA, nor as protein, nor as viral particles.
Conclusions: Islet cells from German landrace pigs do not express PERVs and may therefore be used for breeding genetically modified pigs suitable for xenotransplantation and treatment of diabetes.
Keywords: Xenotransplantation, Diabetes , Porcine Endogenous Retroviruses (PERV), islet cells
29 March 2023 : EditorialEditorial: Effective Direct-Acting Antiviral Treatments Support Global and National Programs to Eliminate H...
Med Sci Monit In Press; DOI: 10.12659/MSM.940519
06 December 2022 : Original articleChanges in Physical Activity Due to Fear of COVID-19 and Its Impact on Depression Among Post-Liver Transpla...
Ann Transplant 2022; 27:e938239
04 October 2022 : Original articleIncidence of COVID-19 and Identification of Possible Risk Factors Associated with COVID-19 in Acute Renal T...
Ann Transplant 2022; 27:e937688
12 April 2022 : Letter/CorrespondencePancreas Allograft Thrombosis as a Post-COVID-19 Complication in a Diabetic Patient After Pancreas Transpla...
Ann Transplant 2022; 27:e935863
27 Mar 2023 : Original articleOutcomes of Kidney Transplantation in Older Recipients
Ann Transplant In Press; DOI: 10.12659/AOT.938692
27 Mar 2023 : Original articleThe Incidence of Brain-Dead Donors Based on Screening and Management Led by Intensivists
Ann Transplant In Press; DOI: 10.12659/AOT.939521
22 Mar 2023 : Original articleEffect of Sodium Bicarbonate Ringer’s Solution on Intraoperative Blood Gas Analysis and Postoperative Recov...
Ann Transplant In Press; DOI: 10.12659/AOT.939097
17 Mar 2023 : Original articleConverting from Tenofovir Disoproxil Fumarate to Tenofovir Alafenamide in Patients with Hepatitis B Followi...
Ann Transplant In Press; DOI: 10.12659/AOT.938731
Most Viewed Current Articles
24 Aug 2021 : Review articleNormothermic Machine Perfusion (NMP) of the Liver – Current Status and Future Perspectives
Ann Transplant 2021; 26:e931664
26 Jan 2022 : Review articleRecurrence of Hepatocellular Carcinoma After Liver Transplantation: Risk Factors and Predictive Models
Ann Transplant 2022; 27:e934924
29 Dec 2021 : Original articleEfficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...
Ann Transplant 2021; 26:e933588
15 Mar 2022 : Case reportCombined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...
Ann Transplant 2022; 27:e935860