Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

21 May 2009

What else differs cellular rejection grade 1a from 0? annexin v and bcl in elective biopsies received from heart transplant recipients

S Zeglen, J Nozynski, E Wozniak-Grygiel, M Zakliczynski, A Laszewska, M Zembala

Ann Transplant 2009; 14(1): 31-31 :: ID: 880312

Abstract

Background: Despite of morphologic differences between non-rejection (0 due to ISHLT scale) and moderate focal cellular rejection (1a, ISHLT) i.e.  lymphocytes aggregation, the genetic and clinical differences in CARGO studies were not shown. Therefore the aim of the study was to compare the expression of selected antigens associated with apoptosis in heart transplant (HTx) recipients in the context of 0 and 1a cellular rejection. The expression of Annexin V, Bcl-2 (protective apoptotic activity), Bcl-xL (antiapoptotic activity) and Bcl-xS/L (other antiapoptotic mechanism) were assessed.
Material/Methods: 17 heart transplant patients were retrospectively included  into the trial (2 women and 15 men, mean age 46.2±13.9 yr, BMI 25.7±3.2). 10 biopsies presented rejection 0 and next 10-1a (due to ISHLT scale) - group A and B, respectively. Endomyocardial biopsy specimens were processed using routine immunohistochemical method. The frozen sections were incubated with adequate anti-human antibodies from BioVision and Santa Cruz Biotechnology. The expression was assessed according to IHC method i.e. 0 - the lack of expression, 1 - trace, 2 - distinct and 3 - strong. The correlation was analyzed between particular molecules expression. Results: The significant increase of Bcl-2 expression together with rejection was observed. The expression of other antigens was also shown but without signs of any significant tendency. No correlation in group A was noted, on the contrary, in group B the significant strong and negative correlation between Bcl-2 and Bcl-xS/L was revealed.
Conclusions: Bcl-2 expression responds to the morphologic progression of
graft rejection and is opposing to Bcl-xS/L activity.

Keywords: Heart Transplantation

Add Comment 0 Comments

In Press

06 Feb 2024 : Case report  

Successful Sequential Liver and Isolated Intestine Transplantation for Mitochondrial Neurogastrointestinal ...

Ann Transplant In Press; DOI: 10.12659/AOT.941881  

12 Feb 2024 : Original article  

No Prognostic Impact of Graft-to-Recipient Weight Ratio on Hepatocellular Carcinoma Recurrence Following Li...

Ann Transplant In Press; DOI: 10.12659/AOT.942767  

21 Feb 2024 : Original article  

Use of LCP-Tacrolimus (LCPT) in Kidney Transplantation: A Delphi Consensus Survey of Expert Clinicians

Ann Transplant In Press; DOI: 10.12659/AOT.943498  

Most Viewed Current Articles

05 Apr 2022 : Original article  

Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver Transplantation

DOI :10.12659/AOT.935604

Ann Transplant 2022; 27:e935604

12 Jan 2022 : Original article  

Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...

DOI :10.12659/AOT.934738

Ann Transplant 2022; 27:e934738

22 Nov 2022 : Original article  

Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...

DOI :10.12659/AOT.937988

Ann Transplant 2022; 27:e937988

15 Mar 2022 : Case report  

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358