Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

21 May 2009

Tissue fluid/lymph cytokines and growth factors regulate human keratinocyte proliferation and differentiation

A Domaszewska, M Zaleska, W Olszewski

Ann Transplant 2009; 14(1): 36-36 :: ID: 880328


Background: Our previous studies revealed presence of a number of growth
factors and cytokines in human skin tissue fluid/lymph (TF/L) at levels higher than in serum. This prompted us to study whether TF/L may have a regulatory effect on keratinocyte (KC) growth. Aim: To study the effect of TF/L on proliferation and differentiation of KC and their stem cell markers expression.
Material/Methods: KC were isolated from lower limb skin and were cultured
for 1 to 7 days in TF/L. Phenotypes were identifi ed using antibodies against
p63, CD29, Ki67 and PCNA. Blocking of cytokines with antibodies helped to
estimate which cytokine stimulated KC proliferation and differentiation.
Results: KC cultured in TF/L showed higher than in controls percentage of
dividing and elongated cells from basal layer as well as lower percentage of
differentiated cells from upper layers. Higher percentage of p63 and CD29
positive cells was also observed. Neutralization of IL-1b, IL-6, TNF-a, KGF caused decrease in percentage of mitotic cells. Neutralization of KGF decreased percentage of p63 and CD29 positive cells.
Conclusions: TF/L cytokines have a stimulating effect on proliferation of basal
KC but not on their differentiation. KGF turned to be a strong stimulator.

Keywords: biostatic grafts, composite tissue transplantation, Limb Transplantation

Add Comment 0 Comments

In Press

12 Feb 2024 : Original article  

No Prognostic Impact of Graft-to-Recipient Weight Ratio on Hepatocellular Carcinoma Recurrence Following Li...

Ann Transplant In Press; DOI: 10.12659/AOT.942767  

21 Feb 2024 : Original article  

Use of LCP-Tacrolimus (LCPT) in Kidney Transplantation: A Delphi Consensus Survey of Expert Clinicians

Ann Transplant In Press; DOI: 10.12659/AOT.943498  

Most Viewed Current Articles

05 Apr 2022 : Original article  

Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver Transplantation

DOI :10.12659/AOT.935604

Ann Transplant 2022; 27:e935604

12 Jan 2022 : Original article  

Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...

DOI :10.12659/AOT.934738

Ann Transplant 2022; 27:e934738

22 Nov 2022 : Original article  

Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...

DOI :10.12659/AOT.937988

Ann Transplant 2022; 27:e937988

15 Mar 2022 : Case report  

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358