21 May 2009
The influence of cyclosporine a and rapamycin on the expression of CD11b and CD57 within CD8+CD28- population in renal allograft recipients
A Korecka-Polak, P Wierzbicki, A Duszota, K Bocian, M Niemczyk, D Klosowska, J Wyzgal, L Paczek, A Chmura, M Durlik, A Gorski, G Korczak-KowalskaAnn Transplant 2009; 14(1): 37-38 :: ID: 880334
Abstract
Background: The function of the allograft seems to be dependent on the level of regulatory lymphocytes including CD8+CD28- cells. This is a heterogonous population containing both suppressor (Ts) and cytotoxic cells (Tc). It is possible that adhesive molecules CD11b and CD57 can be helpful to distinguish between Ts and Tc subpopulations. The aim of this study was to evaluate the differences in the expression of CD11b and CD57 within CD8+CD28- population in renal allograft recipients with different immunosuppressive regimens (CsA or RAPA) and different graft's function.
Material/Methods: Peripheral blood was obtained from the healthy volunteers
and patients divided into four groups: CsA-Sta, RAPA-Sta, CsA-CR, RAPA-CR.
Sta - stable graft function; CR - chronic graft rejection; CsA - cyclosporine A,
azathioprine, prednisone; RAPA - rapamycin, prednisone. Peripheral blood
mononuclear cells were labelled with mouse anti-human mAbs conjugated with fluorochromes and analyzed by flow cytometry (FACSCalibur).
Results: In CsA-Sta group the expression of CD11b within CD8+CD28- population was lower than in the healthy volunteers. The expression of CD57 within CD8+CD28- population in RAPA-Sta group was higher than in the healthy volunteers, CsA-Sta and CsA-CR groups. In the group with the biggest variation in the number of HLA donor/recipients mismatches - CsA-CR, we observed some correlations between the number of HLA mismatches and the expression of CD11b and CD57 within CD8+CD28- population: positive for CD57 and HLA-DR, negative for CD11b and HLA-A, positive for CD11b and HLA-B.
Conclusions: The expression of CD11b and CD57 within CD8+CD28- population in renal allograft recipients seems to be dependent not only on the type of immunosuppressive regimen but also on the number of HLA mismatches between the donor and recipient. Therefore both of these factors can influence the ratio Ts: Tc in heterogonous CD8+CD28- population.
Keywords: Immunosuppression, Kidney Transplantation
In Press
15 Mar 2024 : Review article
Approaches and Challenges in the Current Management of Cytomegalovirus in Transplant Recipients: Highlighti...Ann Transplant In Press; DOI: 10.12659/AOT.941185
18 Mar 2024 : Original article
Does Antibiotic Use Increase the Risk of Post-Transplantation Diabetes Mellitus? A Retrospective Study of R...Ann Transplant In Press; DOI: 10.12659/AOT.943282
20 Mar 2024 : Original article
Transplant Nephrectomy: A Comparative Study of Timing and Techniques in a Single InstitutionAnn Transplant In Press; DOI: 10.12659/AOT.942252
28 Mar 2024 : Original article
Association Between FEV₁ Decline Rate and Mortality in Long-Term Follow-Up of a 21-Patient Pilot Clinical T...Ann Transplant In Press; DOI: 10.12659/AOT.942823
Most Viewed Current Articles
05 Apr 2022 : Original article
Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver TransplantationDOI :10.12659/AOT.935604
Ann Transplant 2022; 27:e935604
12 Jan 2022 : Original article
Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...DOI :10.12659/AOT.934738
Ann Transplant 2022; 27:e934738
22 Nov 2022 : Original article
Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...DOI :10.12659/AOT.937988
Ann Transplant 2022; 27:e937988
15 Mar 2022 : Case report
Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...DOI :10.12659/AOT.935860
Ann Transplant 2022; 27:e935860