Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

21 May 2009

Monitoring of microchimerism and anti-hla antibodies in the first polish recipient of hand transplantation in the early post-transplantation period

W Niepiekło, A Chełmoński, M Kamińska, J Jabłecki, D Patrzałek, B Nowakowska

Ann Transplant 2009; 14(1): 68-68 :: ID: 880451


Background: The hand transplant consists of different tissues of varying immunogenetic potential. Donor's cells can contribute to either rejection or tolerance of the graft. The aim of our study was monitoring of anti-HLA antibodies, a mark of humoral rejection, and microchimerism (induction of donor specific tolerance?) in the first 3 months after transplantation.
Material/Methods: The recipient was 34 year old man with blood group A- positive; HLA-phenotype A24; B18, 60; DRB1*11. Multi organ cadaveric donor was 41 year-old man blood group A-positive; HLA-phenotype A 31,32; B 35, 39; DRB1*11,16, negative T- and B-cell cross-match. We examined recipient's blood and sera specimens, obtained 1 and 2 weeks, and 1, 2 and 3 months after transplantation. Anti-HLA antibodies were monitored by the complement-dependent cytotoxicity assay (CDC), with prolonged time of incubation, with PBL and B lymphocyte. Microchimerism was analyzed using nested polymerase chain reaction with sequence specific primers typing methods (nested
PCR-SSP) for detection of HLA-A alleles.
Results: No anti-HLA alloantibodies were detected in the study period, what
confirmes that humoral rejection occurs rarely, if at all, in the human composite tissue allografts. Peripheral microchimerism was observed in all tested specimens. It is still debated whether this microchimerism can induce tolerance or is it only a side effect of transplantation.

Keywords: composite tissue transplantation, Limb Transplantation


Most Viewed Current Articles

26 Jan 2022 : Review article  

Recurrence of Hepatocellular Carcinoma After Liver Transplantation: Risk Factors and Predictive Models

DOI :10.12659/AOT.934924

Ann Transplant 2022; 27:e934924

24 Aug 2021 : Review article  

Normothermic Machine Perfusion (NMP) of the Liver – Current Status and Future Perspectives

DOI :10.12659/AOT.931664

Ann Transplant 2021; 26:e931664

29 Dec 2021 : Original article  

Efficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...

DOI :10.12659/AOT.933588

Ann Transplant 2021; 26:e933588

15 Mar 2022 : Case report  

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358