Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

21 May 2009

Molecular epidemiology of vancomycin resistant enterococcus faecium infecting solid organs recipients in the transplantology and transplant surgery wards in 2005–2006

G Młynarczyk, A Mrówka, W Grzybowska, S Tyski, T Buczkowska, J Pazik, M Durlik, L Adadyński, A Kwiatkowski, A Chmura, L Pączek, A Młynarczyk

Ann Transplant 2009; 14(1): 70-71 :: ID: 880459


Background: Because of the significant increase of the number of vancomycin resistant enterococci (VRE), especially Enterococcus faecium isolated from patients of Institute of Transplantology, It seems necessary to investigate the molecular epidemiology of these strains.
Material/Methods: VRE (E. faecium) isolated from various specimens from patients of Institute of Transplantology from 01.01.2005 to 31.12.2006 were investigated. After initial identification strains recognized as VRE were frozen and stored in -70°C until further testing. The strains were examined in the PCR reaction for the presence of vanA, vanB, vanD and vanG genes as well as ddl gene, to confirm type of vancomycin resistance and correctness of identification. Confirmed VRE E. faecium strains were examined by the molecular method known as restriction fragments length polymorphism - pulsed field - gel electrophoresis (RLFP-PFGE). Only one isolate per one patient was analyzed.
Results: In the investigated two years period, 57 patients of Institute of Transplantology were infected with VRE. 26 of them were recipients of liver, 22 of kidney and remaining 9 were other surgery or nephrology patients. All VRE strains from 2005 and the most from 2006 possessed vanA gene, only 5 strains possessed the vanB determinant. The PFGE analysis revealed among strains possessing vanA determinant a large group of related strains and several small groups as well as unrelated strains. Strains possessing vanB determinant were unrelated.
Conclusions: The PFGE analysis enables the investigation of epidemiology of
VRE among patients after transplantations. Strains with similar patterns most
probably originated from the one source and it may suggest an outbreak.

Keywords: Molecular

Add Comment 0 Comments

In Press

12 Feb 2024 : Original article  

No Prognostic Impact of Graft-to-Recipient Weight Ratio on Hepatocellular Carcinoma Recurrence Following Li...

Ann Transplant In Press; DOI: 10.12659/AOT.942767  

21 Feb 2024 : Original article  

Use of LCP-Tacrolimus (LCPT) in Kidney Transplantation: A Delphi Consensus Survey of Expert Clinicians

Ann Transplant In Press; DOI: 10.12659/AOT.943498  

Most Viewed Current Articles

05 Apr 2022 : Original article  

Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver Transplantation

DOI :10.12659/AOT.935604

Ann Transplant 2022; 27:e935604

12 Jan 2022 : Original article  

Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...

DOI :10.12659/AOT.934738

Ann Transplant 2022; 27:e934738

22 Nov 2022 : Original article  

Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...

DOI :10.12659/AOT.937988

Ann Transplant 2022; 27:e937988

15 Mar 2022 : Case report  

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358