21 May 2009
Differences in antiapoptotic and proliferative mechanisms in ischemic and dilatative cardiomyopathy – study of hearts explanted from transplant
J Nożyński, M Zakliczyński, D Konecka-Mrówka, D Lange, E Zembala-Nożyńska, T Męcik-Kronenberg, K Dąbrówka, S Żegleń, R Przybylski, M ZembalaAnn Transplant 2009; 14(1): 31-32 :: ID: 880521
Abstract
Background: Antiapoptotic and proliferative mechanisms take place at cardiocytes' death in dilatative cardiomyopathy (DCM), ischemic heart disease (IHD) and myocardial remodeling by the mitochondrial pathway. This process is enhanced mainly by Bcl-2 protein. We sought, to estimate Bcl-2 antiapoptotic expression and Ki-67 proliferative activity in cardiomyopathies.
Material/Methods: The study groups included 7 hearts with DCM and 8 hearts with IHD explanted during transplantation. The comparative group consisted of myocardial hypertrophy. The control group were donor fragments. Bcl-2 expression was determined immunohistochemically by NP030 antibody (DAKO), whereas proliferation was detected with M7240 antibody. The reacion was enhanced with Envision-DAB in histologic slides.. The intensity of staining was assessed semiquantitatively.
Results: No Bcl-2 expression was seen in the controls, the strongest reaction was seen in DCM group (mean range 2.71), then in IHD (mean range 1.75) and in the comparative group, myocardial hypertrophy (mean range 0.29). The statistical comparisons showed no differences between control and the comparative group, whereas other comparisons showed statistical significance. The proliferative activity was seen only in endocardial and interstitial fibroblasts in cases with DCM and IHD with no reaction in myocytes in any group. Conclusions: Antiapoptotic Bcl-2 activity plays an important role in cardiocyte preservation, while proliferative activity is resticted to interstitial connective tissue cells as replacement process. Myocardial Bcl-2 expression may serve as a diagnostic tool in differentiation of cardiomyopathies and as an indicator of active myocardial injury.
Keywords: Heart Transplantation
In Press
Case report
Pulmonary Embolism Following Living Donor Hepatectomy: A Report of 4 Cases and Literature ReviewAnn Transplant In Press; DOI: 10.12659/AOT.946752
Most Viewed Current Articles
03 Jan 2023 : Original article 6,527
Impact of Autologous Stem Cell Transplantation on Primary Central Nervous System Lymphoma in First-Line and...DOI :10.12659/AOT.938467
Ann Transplant 2023; 28:e938467
15 Aug 2023 : Review article 6,222
Free-Circulating Nucleic Acids as Biomarkers in Patients After Solid Organ TransplantationDOI :10.12659/AOT.939750
Ann Transplant 2023; 28:e939750
16 May 2023 : Original article 6,152
Breaking Antimicrobial Resistance: High-Dose Amoxicillin with Clavulanic Acid for Urinary Tract Infections ...DOI :10.12659/AOT.939258
Ann Transplant 2023; 28:e939258
17 Jan 2023 : Original article 5,279
Non-Cryopreserved Peripheral Blood Stem Cell Graft for Autologous Hematopoietic Stem Cell Transplantation i...DOI :10.12659/AOT.938595
Ann Transplant 2023; 28:e938595