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29 June 2012

Comparing the effect of Biolasol® and HTK solutions on maintaining proper homeostasis, indicating the kidney storage efficiency prior to transplantation

Barbara DolińskaACEG, Aneta Ostróżka-CieślikBCEFG, Artur CabanADE, Lech CierpkaD, Florian RyszkaADF

DOI: 10.12659/AOT.883225

Ann Transplant 2012; 17(2): 74-78

Abstract

Background: Maintaining proper homeostasis involving normal physiological level of extra- and intracellular solutions is one of the factors that determine restoring the life functions of a transplanted organ. The aim of this study was to demonstrate the effectiveness of the newly developed Biolasol® solution in kidney storage and to compare its protective effect to the standard HTK solution.
Material/Methods: Isolated porcine kidneys were perfused, preserved (24 and 48 h) and reperfused with Biolasol® and HTK solutions. The perfusate samples were used to analyze pH; the amount of released indicator enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH); and the concentration of sodium, potassium and magnesium.
Results: Kidney storage in the HTK liquid may cause metabolic acidosis after 24 and 48 hour preservation (pH drop below 6.8). pH of perfusates sampled after perfusion and reperfusion with Biolasol® solution was within the range 6.8–7.7. The content of sodium ions during perfusion and reperfusion was the closest to the reference values while using the Biolasol® solution. Only Biolasol® ensured normal homeostasis of Mg2+ ions. In the presence of the HTK solution their level was significantly (more than 1000%) higher than the normal physiological value. For both solutions, ALT and AST activities were within the normal range or differed only slightly.
Conclusions: Biolasol® and HTK solutions protect kidneys against ischemic damage. Still, Biolasol® offers better homeostasis maintenance, which may suggest it more effectively preserves kidneys prior to transplantation.

Keywords: Biolasol, HTK, Kidney, Preservation

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Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358