25 October 2013 : Original article
Porcine endogenous retrovirus infection changes the expression of inflammation-related genes in lipopolysaccharide-stimulated human dermal fibroblasts
Magdalena C. KimsaADE, Barbara Strzałka-MrozikADE, Małgorzata W. KimsaCDF, Celina Kruszniewska-RajsBDF, Joanna GolaAE, Jolanta AdamskaBF, Antoni RajsDE, Urszula MazurekAGDOI: 10.12659/AOT.889310
Ann Transplant 2013; 18:576-586
Abstract
BACKGROUND: The present study focuses on explaining the interaction between porcine endogenous retroviruses (PERVs) and human cells in inflammatory conditions. The differences in expression of selected inflammation-related genes in human dermal fibroblasts (NHDF) infected with PERVs with and without lipopolysaccharide stimulation were identified.
MATERIAL AND METHODS: The PERV infectivity was analyzed using a co-culture of NHDF and PK15 cells. Quantification of PERV A, B DNA and PERV A, B RNA was performed by real-time QPCR and QRT-PCR. The analysis of the expression profile was performed using HG-U133A 2.0 oligonucleotide microarrays.
RESULTS: PERV infection of NHDF cells with LPS stimulation resulted in a statistically significant decrease in the copy number of PERV A DNA, and an increase in the copy number of PERV A RNA compared to fibroblasts without stimulation. There was no statistically significant difference between the copy number of PERV B RNA of LPStreated and untreated NHDF cells. Typing of differentiation genes was performed in a panel of 571 selected transcripts of inflammation-related genes. Among all studied genes, 23 were differentially regulated with a change greater that 1.1-fold and p<0.05 in all studied groups. Of these 23 genes, 3 were found to be regulated by more than 2.0-fold at least in 2 studied groups (IL6, IL8, and IL33).
CONCLUSIONS: The interaction between porcine endogenous retroviruses and human cells changes in inflammatory conditions. PERV infection of NHDF cells may alter the expression of inflammation-related genes. Further investigations concerning PERV infection of human cells in different conditions seem to be necessary.
Keywords: porcine endogenous retrovirus, lipopolysaccharide, Xenotransplantation, oligonucleotide microarray, quantitative PCR
SARS-CoV-2/COVID-19
29 March 2023 : Editorial
Editorial: Effective Direct-Acting Antiviral Treatments Support Global and National Programs to Eliminate H...Med Sci Monit In Press; DOI: 10.12659/MSM.940519
06 December 2022 : Original article
Changes in Physical Activity Due to Fear of COVID-19 and Its Impact on Depression Among Post-Liver Transpla...Ann Transplant 2022; 27:e938239
04 October 2022 : Original article
Incidence of COVID-19 and Identification of Possible Risk Factors Associated with COVID-19 in Acute Renal T...Ann Transplant 2022; 27:e937688
12 April 2022 : Letter/Correspondence
Pancreas Allograft Thrombosis as a Post-COVID-19 Complication in a Diabetic Patient After Pancreas Transpla...Ann Transplant 2022; 27:e935863
In Press
27 Mar 2023 : Original article
Outcomes of Kidney Transplantation in Older RecipientsAnn Transplant In Press; DOI: 10.12659/AOT.938692
27 Mar 2023 : Original article
The Incidence of Brain-Dead Donors Based on Screening and Management Led by IntensivistsAnn Transplant In Press; DOI: 10.12659/AOT.939521
22 Mar 2023 : Original article
Effect of Sodium Bicarbonate Ringer’s Solution on Intraoperative Blood Gas Analysis and Postoperative Recov...Ann Transplant In Press; DOI: 10.12659/AOT.939097
17 Mar 2023 : Original article
Converting from Tenofovir Disoproxil Fumarate to Tenofovir Alafenamide in Patients with Hepatitis B Followi...Ann Transplant In Press; DOI: 10.12659/AOT.938731
Most Viewed Current Articles
24 Aug 2021 : Review article
Normothermic Machine Perfusion (NMP) of the Liver – Current Status and Future PerspectivesDOI :10.12659/AOT.931664
Ann Transplant 2021; 26:e931664
26 Jan 2022 : Review article
Recurrence of Hepatocellular Carcinoma After Liver Transplantation: Risk Factors and Predictive ModelsDOI :10.12659/AOT.934924
Ann Transplant 2022; 27:e934924
29 Dec 2021 : Original article
Efficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...DOI :10.12659/AOT.933588
Ann Transplant 2021; 26:e933588
15 Mar 2022 : Case report
Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...DOI :10.12659/AOT.935860
Ann Transplant 2022; 27:e935860