12 March 2014 : Original article
Impact of donor health on corneal biochemistry – an unexpected caveat from a pilot study
Tomasz KryczkaACDEF, Stanisław J. ChrapustaCDE, Jacek P. SzaflikBF, Jerzy SzaflikAB, Anna MidelfartDEFGDOI: 10.12659/AOT.890215
Ann Transplant 2014; 19:129-137
Abstract
BACKGROUND: To test the possibility that some chronic systemic maladies not directly related to the function of the eye may significantly and permanently disturb corneal metabolism.
MATERIAL AND METHODS: Contents of selected low molecular weight metabolites were compared among corneas collected from donors who died suddenly of an accident or non-poisoning suicide, or met a sudden non-accidental death from unidentified causes, or died of a chronic cardiovascular disease or of idiopathic liver cirrhosis (N=4 for each group). Corneal buttons were halved; one half was snap-frozen and stored at –80°C, and the other half was stored at +4°C in Eusol-C for 8 days and then was snap-frozen and stored at –80°C until analyzed. Metabolite contents were assessed using high-resolution magic angle spinning proton NMR spectroscopy.
RESULTS: Significant between-group differences in corneal biochemical profiles were identified. Most of them were reduced or nullified by the Eusol-C storage, suggesting their link to differences in in vivo corneal environment. The corneas from donors with liver cirrhosis or cardiovascular diseases differed considerably from the remaining ones, both before and after the Eusol-C storage.
CONCLUSIONS: Various chronic systemic diseases that are not directly related to the function of the eye markedly affect corneal biochemistry. Some of the alterations are likely related to a permanent aberration in corneal metabolism. A study is warranted in larger donor groups on the effect of idiopathic liver cirrhosis and cardiovascular diseases on corneal metabolism and/or a retrospective analysis of the long-term outcome of keratoplasty and other grafting procedures employing materials from these donor groups.
Keywords: cornea biochemistry, cornea grafting, cold storage, Metabolomics, NMR spectroscopy, Cause of Death
698 7
In Press
14 Sep 2023 : Case report
Intrahepatic Venous Collateral Circulation and Reverse Blood Flow After Main Hepatic Vein Obstruction: A Ca...Ann Transplant In Press; DOI: 10.12659/AOT.941521
12 Sep 2023 : Original article
Three-Dimensional Printing of Polycaprolactone/Nano-Hydroxyapatite Composite Scaffolds with a Pore Size of ...Ann Transplant In Press; DOI: 10.12659/AOT.940365
04 Sep 2023 : Original article
Analysis of Anxiety and Influencing Factors in Kidney Transplant Recipients and General Public During the C...Ann Transplant In Press; DOI: 10.12659/AOT.941489
04 Sep 2023 : Original article
Prolonged Tracheal Intubation in the ICU as a Possible Risk Factor for Arytenoid Dislocation After Liver Tr...Ann Transplant In Press; DOI: 10.12659/AOT.940727
Most Viewed Current Articles
24 Aug 2021 : Review article
Normothermic Machine Perfusion (NMP) of the Liver – Current Status and Future PerspectivesDOI :10.12659/AOT.931664
Ann Transplant 2021; 26:e931664
26 Jan 2022 : Review article
Recurrence of Hepatocellular Carcinoma After Liver Transplantation: Risk Factors and Predictive ModelsDOI :10.12659/AOT.934924
Ann Transplant 2022; 27:e934924
29 Dec 2021 : Original article
Efficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...DOI :10.12659/AOT.933588
Ann Transplant 2021; 26:e933588
15 Mar 2022 : Case report
Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...DOI :10.12659/AOT.935860
Ann Transplant 2022; 27:e935860