04 December 2014 : Original article
Enhanced Osteogenic Activity of Mesenchymal Stem Cells and Co-Modified BMP-2 and bFGF Genes
Yi YangACDE, Gele JinBCD, Liang LiAB, XiaoJing LiuCD, Cao LiDFG, Jiang WuEFGDOI: 10.12659/AOT.892461
Ann Transplant 2014; 19:629-638
Abstract
BACKGROUND: We co-modified rat mesenchymal stem cells (rMSCs) with bone morphogenetic protein-2 (BMP-2) and basic fibroblast growth factor (bFGF) genes, and then evaluated their osteogenic activity in vitro and evaluated ectopic bone formation in vivo.
MATERIAL AND METHODS: Nude mice (male, 20±2 g) were randomly divided into 2 groups: a control group (n=30) and a transfection group (n=30). In the control group, rMSCs+scaffold was implanted into the intramuscular pocket in the left femur, and BMP-2 gene modified rMSCs+scaffold was implanted into the intramuscular pocket in the right femur. In the transfection group, bFGF gene modified rMSCs+scaffold was implanted into the left femur, and BMP-2+bFGF gene modified rMSCs+scaffold was implanted into the right femur. Under anesthesia, an 8-mm incision was made over the lateral aspect of each femur and cell-scaffold composite was implanted into an intramuscular pocket created by blunt dissection. Samples taken at 3 months were stained. Sections were counterstained with hematoxylin. The relative capillary density was estimated by counting the capillary number and measuring the length of the capillaries in the defined area after image digitization.
RESULTS: Comparison with the BMP-2 group and bFGF group, the proliferation of the BMP-2+bFGF group was obviously enhanced (p<0.01). The modification could promote alkaline phosphatase activity in endochylema of MSCs in BMP-2+bFGF group. Osteocalcin secretion had obviously improved in the BMP-2+bFGF group compared with the BMP-2 group and the bFGF group (p<0.01). Histologic analysis of the stages of bone formation showed a more obvious scaffold substitution progress in the BMP-2+bFGF group.
CONCLUSIONS: Co-modification of rMSCs with BMP-2 and bFGF genes can promote the in vitro osteogenic activity of rMSCs and in vivo ectopic bone formation.
Keywords: Bone Morphogenetic Protein 2, Fibroblast Growth Factors, Mesenchymal Stem Cell Transplantation
In Press
Original article
Retrospective Study to Compare Outcomes in 159 Patients Undergoing First Autologous Stem Cell Transplantati...Ann Transplant In Press; DOI: 10.12659/AOT.947186
Case report
Pulmonary Embolism Following Living Donor Hepatectomy: A Report of 4 Cases and Literature ReviewAnn Transplant In Press; DOI: 10.12659/AOT.946752
Most Viewed Current Articles
03 Jan 2023 : Original article 6,513
Impact of Autologous Stem Cell Transplantation on Primary Central Nervous System Lymphoma in First-Line and...DOI :10.12659/AOT.938467
Ann Transplant 2023; 28:e938467
15 Aug 2023 : Review article 6,136
Free-Circulating Nucleic Acids as Biomarkers in Patients After Solid Organ TransplantationDOI :10.12659/AOT.939750
Ann Transplant 2023; 28:e939750
16 May 2023 : Original article 6,130
Breaking Antimicrobial Resistance: High-Dose Amoxicillin with Clavulanic Acid for Urinary Tract Infections ...DOI :10.12659/AOT.939258
Ann Transplant 2023; 28:e939258
17 Jan 2023 : Original article 5,257
Non-Cryopreserved Peripheral Blood Stem Cell Graft for Autologous Hematopoietic Stem Cell Transplantation i...DOI :10.12659/AOT.938595
Ann Transplant 2023; 28:e938595