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03 December 2015 : Original article  

Real-Time PCR Analysis of Chimerism in T Cell Subsets as an Early Predictor of Graft-Versus-Host Disease Following Allogeneic Stem Cell Transplantation

Katarzyna GuzABCDEF, Barbara NasiłowskaBDEF, Agnieszka TomaszewskaABDEF, Agnieszka OrzińskaABCD, Justyna Smolarczyk-WodzyńskaBC, Magdalen KrzemienowskaAB, Kazimierz HałaburdaBDE, Maciej PrzybylskiC, Wiesław Wiktor JędrzejczakD, Bożena MariańskaDG, Ewa BrojerABDEFG

DOI: 10.12659/AOT.894621

Ann Transplant 2015; 20:720-728


BACKGROUND: Graft-versus-host-disease (GvHD) is the major cause of morbidity and mortality after stem cell transplantation. The development of early prediction methods is therefore of importance. Our aim was to analyze the usefulness of early donor chimerism monitoring after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in T cells and in CD4+ and CD8+ (lineage chimerism) for GvHD prediction.

MATERIAL AND METHODS: Chimerism was analyzed in 76 consecutive adult patients using RQ-PCR TaqMan technology on DNA extracted from Pan T, CD4+, and CD8+ cell subsets on Day 5, 10, 15 and 30 after allo-HSCT.

RESULTS: The threshold of chimerism predictive for GvHD was the same for all tested cell subsets. In acute myeloid leukemia (AML) patients treated with myeloablative conditioning (MAC), the threshold predictive for acute graft versus host disease was 95% and 99% for Day 10 and Day 15, respectively. In patients treated with reduced intensity conditioning (RIC), the threshold predictive for chronic graft versus host disease was 98% on Day 10. The differences were statistically significant.

CONCLUSIONS: Chimerism analysis in T cell subsets by RQ-PCR on Day 10 and Day 15 after transplantation is useful for prediction of aGvHD (AML patients after MAC) and cGvHD (patients after RIC). However, there was no difference in the results between chimerism in the T cell subsets. Our RQ-PCR protocol was highly sensitive and proved effective for analysis of lineage chimerism.

Keywords: CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Chimerism, Graft vs Host Disease, Real-Time Polymerase Chain Reaction, T-Lymphocytes

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Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358