Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

12 November 2015 : Original article  

Induction and Maintenance of Anti-HBs in Immunosuppressed Rats After Liver Transplantation with HBsAg-pulsed Dendritic Cell Complex

Yakun WuE, Yiming LiuB, Jianping GongF, Shengwei LiD, Wei ZhangAC

DOI: 10.12659/AOT.895028

Ann Transplant 2015; 20:676-682

Abstract

BACKGROUND: This study aimed to investigate the efficacy of hepatitis B surface antigen (HBsAg)-pulsed dendritic cell (DC) complex in the induction and maintenance of anti-HBs in immunosuppressed rats after liver transplantation.

MATERIAL AND METHODS: Lewis-Brown Norway (BN) rat liver transplantation models were successfully established. Recipients were injected with tacrolimus (2 mg/Kg) daily post-operation for three months to maintain immunosuppression state; the recipients were then randomly divided into two groups: HBsAg-DC group (n=15) comprised rats intraperitoneally injected with HBsAg-DC complex at 14 and 28 d post-surgery and HBsAg group (n=15) comprised rats injected with HBsAg (200 μL) once a week for 12 weeks. Untreated rats post-transplantation were included in the control group (n=5). Histopathological changes were detected by light microscopy and transmission electron microscopy; mRNA expressions of IL-2 and IFN-γ in graft liver were analyzed through real-time polymerase chain reaction. Serum IL-2 and IFN-γ levels and anti-HB titer were detected through enzyme-linked immunosorbent assay. Changes in CD4+ and CD8+ T cells in the blood were detected through flow cytometry.

RESULTS: IL-2 and IFN-γ expressions were lower in HBsAg-DC and HBsAg groups than in the control group (P<0.05). A high FK506 dose also induced a milder allograft rejection than the control dose. These findings showed that a high FK506 dose caused immunosuppression in rats after liver transplantation. A high anti-HB titer was detected in the HBsAg-DC group in one, two, and three months post-operation; by contrast, anti-HB titer was barely detected in the HBsAg group.

CONCLUSIONS: High anti-HB titers could be induced and maintained in immunosuppressed rats; therefore, HBsAg-DC complex may prevent HBV reinfection after recipients undergo liver transplantation.

Keywords: Hepatitis B Antibodies, Hepatitis B virus, Immunosuppression, Liver Transplantation

Add Comment 0 Comments

In Press

Original article  

Biopsychosocial Effects of Donor Traits on Heart Transplant Recipients

Ann Transplant In Press; DOI:  

Clinical Research  

Ten-Year Retrospective Analysis of Continuous Renal Replacement Therapy in Burn Patients: Impact on Surviva...

Ann Transplant In Press; DOI:  

Original article  

Factors Influencing Stress Disorders in Intensive Care Unit (ICU) Patients After Liver Transplantation: A C...

Ann Transplant In Press; DOI: 10.12659/AOT.944320  

Original article  

Hepatopulmonary Syndrome and Liver Transplantation: Impact on Survival and Postoperative Complications

Ann Transplant In Press; DOI: 10.12659/AOT.945297  

Most Viewed Current Articles

05 Apr 2022 : Original article   13,115

Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver Transplantation

DOI :10.12659/AOT.935604

Ann Transplant 2022; 27:e935604

22 Nov 2022 : Original article   10,262

Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...

DOI :10.12659/AOT.937988

Ann Transplant 2022; 27:e937988

12 Jan 2022 : Original article   9,616

Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...

DOI :10.12659/AOT.934738

Ann Transplant 2022; 27:e934738

15 Mar 2022 : Case report   7,277

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358